17 September 2016

Lecture #5: HOST-MICROBE INTERACTION




A process whereby the parasite enters into a relationship with a host is known as infection or disease. In infection or disease, there is an invasion of the body by pathogenic microorganisms. If the disease is transmitted directly or indirectly from one person to another, it is known as communicable disease. A contagious disease is one that is directly transmitted from one person to another.

Types of infection based on how often they occur

1.      Endemic – constantly present in locality (e.g. common cold)

2.      Epidemic – disease occurring in excess of normal (e.g. measles and influenza)

3.      Pandemic – an epidemic that has become worldwide (e.g. pandemic influenza)

4.      Sporadic – if a particular disease occurs only occasionally (e.g. anthrax)


Types of infection according to distribution in the host

1.      Local – the signs and symptoms are confined to one area

e.g. infected wound on the dorsum of the feet

2.      Focal – when the organism involved are originally confined to one area which serves as a continuous source of further dissemination of the organism itself or of toxic materials to other parts of the body (e.g. wound infection caused by C. tetani, this serves as a focus for spread of tetanus toxin throughout the CNS)

3.      Systemic or Generalized – general invasion of the entire body

a.      Bacteremia – when the organism invade the bloodstream with no active multiplication

b.      Septicemia – when the organism invade the bloodstream with active multiplication

c.       Pyemia – when pus–forming organisms repeatedly invade the bloodstream and localize at different parts of the body.

d.     Sapremia – toxic results of the presence in the blood of the product formed by the growth of saprophytes on diseased or injured tissue.

e.      Toxemia – when bacteria are localized in one area, but produce a toxin which spreads and is absorbed by the body cells.


Other types

1.      Primary – initial infection causing the illness (e.g. measles)

2.      Secondary – infections produced by those organisms causing infections only after the primary infection has weakened the body’s defense (e.g. post measles pneumonia)

3.      Mixed – caused by two or more organisms (e.g. wound infection)

4.      Acute – those infection which develop rapidly and usually result in high fever and severe sickness (e.g. whooping cough)

5.      Chronic – those infections which develop with milder but longer lasting symptoms (e.g. tuberculosis)

6.      Inapparent – an illness in which the symptoms are so mild that it goes undetected and thus diagnosed (e.g. poliomyelitis and Hepatitis A)


Essential steps for infection to take place

1.      Infectivity or entrance of the parasite into the host

Fomites – are non–living or inanimate vectors that transmit pathogens

Filth – are polluted materials in the environment which carry microorganisms but are neither true fomites nor insects (e.g. dust, dirt from animal droppings)

Many disease–causing microorganisms have an obligate portal entry and can cause infection only if they enter via such portal (e.g. Shigella bacillus – causes dysentery if it enters by mouth to GIT, has no effect when rubbed into an open wound.

Other microorganisms can enter the body tissues via almost any portal of entry and produce disease (e.g. Pseudomonas aeruginosa)

2.      Invasiveness or establishment and multiplication of the parasite in the host.

From the various portal of entry, they spread:

a.      Directly through the tissue by contiguity.

b.      Via the bloodstream and lymphatics

3.      Perpetuation of species is achieved by

a.      Satisfactory portal of exit from the host

b.      Effective mechanism for transmission of disease (by direct contact, airborne, dust–borne, indirect contact through vectors or inanimate objects)


Phases in the course of infectious diseases

1.      Incubation period – interval between the time the infection received and the appearance of the disease.

2.      Prodromal period – short interval (prodrome) that sometimes follow the period of incubation described by such  ill–defined symptoms as headache or malaise.

3.      Invasion period – disease reaching its full development and maximum intensity.

4.      Fastigum or acne – disease at its height

5.      Defervescence or decline – the stage at which manifestations subside

6.      Convalescent period – the period in which the symptoms may disappear and recovery begins despite the fact that virulent microorganisms are still being discharged from the body.


Attributes of microorganisms which enable them to cause disease

Pathogenicity – ability of the microorganism to produce disease

Two general classes of pathogenic microorganisms

a.      True pathogens – are able to invade the tissues of healthy individuals through some inherent power of their own.

b.      Opportunistic pathogens – are capable of producing disease only when given a special opportunity to enter the body tissues through an accidental injury to the skin or when the natural resistance to infection is abnormally low.

Virulence – the degree of pathogenicity. This is measure in terms of:

a.      Number of microorganisms present

b.      Micrograms of toxins necessary to kill or injure tissue cells.


Factors which influence virulence

1.      Toxic factors

Toxigenicity – ability to produce toxic substances

Toxins – are poisonous substances at high molecular weight produced by pathogenic microorganisms.

Feature                       Exotoxins                              Endotoxins

Bacterial source        excreted by gram (+)            released from the cell wall of
                                                                                    lysed gram (–) bacteria

Chemical nature       protein                                   lipopolysaccharide

Heat intolerance       inactivated easily at             withstand autoclaving
                                    61 – 80oC

Immunology             can be converted to              can not form toxoids, not
                                    Toxoids and readily            neutralized by antitoxins
                                    Neutralized by antitoxins

Pharmacology          specific for a particular       various effects, but mostly
                                    type of cell function             symptoms of generalized
                                                                                    shock/hypersensitivity

Lethal dose               small amount                        larger amount

Microbial disorders caused by exotoxins

a.      Diphtheria (Corynebacterium diptheriae) inhibits protein synthesis which brings about disruption of normal physiologic function

b.      Tetanus (Clostridium tetani) affects synaptic transmission and increases reflex excitability in neurons.

c.       Gas gangrene (Clostridium perfringens) necrotizing and hemolytic, affects also the CNS.

d.     Staphylococcal food poisoning (Staphylococcus aureus) stimulate neutral receptors and increased vomiting within hours.

e.      Cholera (vibrio cholera) produces massive absorption of water and electrolytes.

f.        Streptococcal erythrogenic toxins (Streptococcus pyogenes) produces the typical punctuate maculopapular rash of scarlet fever

2.      Mechanical factors

The multiplication of microorganisms may result in blocking of the capillaries of the heart, lungs and brain.

3.      Miscellaneous factors

a.      Neurotoxin or necrotizing factors – kills tissue cells
b.      Hypothermic factors – lowers body temperature to produce shock
c.       Edema–producing factors

4.      Enzymatic and other related factors

Factors                                   Effects                                                 

Hyaluronidase / Duran       facilitate spread of pathogens       
Reynals Factor)                     and toxic material through
                                                tissues

Coagulase (clotting              causes resistance to phagocytosis
Factor)                                    accelerates formation of blood clot

Hemolysins                           causes lysis of RBC and other tissue cells

Lecithinase or alpha                        similar to hemolysins
            Toxins

Leucodin                               kills leukocyte

Collagenase                          dissolves collagen

Steptokinase             /                       dissolves human fibrin; easily dissolve blood clots
Streptodornase

Capsule                                 resists phagocytosis


Relationship between factors of infection

            D is a function of NV/R      

                        Where:           D – diseases
                                                N – number of disease producing microbes
                                                V – virulence of the organism
                                                R – resistance of the host

The formula, signifies that, in any case of infection, it is the total disease–producing power of the organisms (which depends on their number and virulence), balanced against resistance of the infected individual, that determines the nature and severity of the disease.


  






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